SubtiBank SubtiBank
ycbU [2020-04-07 17:02:55]
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ycbU [2020-04-07 17:02:55]

cysteine desulfurase
locus
BSU_02660
pI
6.45
mw
40.65 kDa
protein length
370 aa Sequence Blast
gene length
1113 bp Sequence Blast
function
unknown
product
cysteine desulfurase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    287,499 288,611

    The protein

    Protein family

  • [SW|Class-V pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|NifZ], [protein|88ACE6B79534338F7C72F107B35A0B2384007088|SufS], [protein|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|NifS], [protein|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|YrvO]

    • [SW|Cofactors]

  • PLP

    • Structure

  • [PDB|5B87] (from Thermococcus onnurineus, corresponds to aa 4 ... 281, 26.5% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|ycbU]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-C039 (ycbU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2037 NBRP B. subtilis, Japan]
  • BKE02660 ([gene|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|ycbU]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATGATTCACTCCTTTA, downstream forward: _UP4_TAAACATGAAAAAGCCCCTG
  • BKK02660 ([gene|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|ycbU]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATGATTCACTCCTTTA, downstream forward: _UP4_TAAACATGAAAAAGCCCCTG